Biosynthesis of Rat serum albumin
نویسندگان
چکیده
منابع مشابه
Biosynthesis of serum albumin in rat liver. Isolation and probable structure of 'proalbumin' from rat liver.
1. Two methods are described for the preparation of 'proalbumin' in good yields from rat liver. 2. One of the methods does not depend on the use of specific antisera. 3. The product from both methods is identical as judged by electrophoresis on polyacrylamide gel, isoelectric focusing on pH gradients, ion-exchange chromatography and quantitative immunoelectrophoresis. The protein also appears t...
متن کاملThe biosynthesis of rat serum albumin. I. Properties of rat albumin and its occurrence in liver cell fractions.
Serum albumin has many characteristics which make it an attractive subject for the study of protein biosynthesis. It is abundant in serum and is produced by the liver at a rapid rate. Its high stability and solubility make for easy isolation. Structurally it appears to consist of a single long chain of about 550 amino acid residues without prosthetic groups. Some data on the formation of chicke...
متن کاملThe Biosynthesis of Rat Serum Albumin I. PROPERTIES OF RAT ALBUMIN AND ITS OCCURRENCE IN LIVER CELL FRACTIONS* THEODORE
Serum albumin has many characteristics which make it an attractive subject for the study of protein biosynthesis. It is abundant in serum and is produced by the liver at a rapid rate. Its high stability and solubility make for easy isolation. Structurally it appears to consist of a single long chain of about 550 amino acid residues without prosthetic groups. Some data on the formation of chicke...
متن کاملThe biosynthesis of rat serum albumin. Composition and properties of the intracellular precursor, proalbumin.
Proalbumin, the intracellular precursor of circulating albumin, is known to contain a basic oligopeptide segment at the N H z terminus. It is not known whether there are other structural differences between albumin and proalbumin, nor is a function of the pro-form recognized. We purified about 25 mg of proalbumin from rat liver microsomes and compared its composition and certain physical and ch...
متن کاملThe biosynthesis of rat serum albumin. In vivo studies on the formation of the disulfide bonds.
In order to learn at what stage the disulfide bonds of albumin are formed during its biosynthesis, we perfused rat livers with iodoacetamide and then isolated the intracellular precursor, proalbumin, from organelles known to be in the pathway of albumin synthesis and secretion. The alkylated cysteines in proalbumin were determined as a measure of its thiol groups in vivo. Proalbumin of smooth m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0306-3283
DOI: 10.1042/bj1230649